The flipr calcium 6 assay kit fluorophore is designed to be better retained within the cell. The flipr calcium 4 assay kit, a nowash, fluorescencebased method used for detecting changes in intracellular calcium concentration which is used for broad spectrum of gpcr targets. Enhance your calcium screens with flipr calcium 6 assay kits. Proprietary chemistry and formulation nowash workflow for highthroughput assays flipr calcium 6 assay kit provides bestinclass data. Carefully follow the thawing and plating instructions, this step is essential for optimal cell survival and. Calcium assays for the flipr tetra system molecular devices. A flipr assay for evaluating agonists and antagonists of gpcr. Intracellular calcium measurements, flipr calcium assay.
Flipr calcium 5 assay kit the flipr calcium 5 assay kit is a homogeneous, fluorescence reagent kit that solves the challenges of using traditional wash method assays, or other nowash dyes with high background signals. Compound effects on calcium transients in pluricyte. The flipr tetra system and the flipr calcium assay kit are used to determine the change in relative light units elicited by carbacholinduced calcium mobilization. Figure 5 illustrates that the flipr calcium 6 assay kit with no probenecid produces a smaller signal, however the assay. Application note flipr calcium assay inscreenex gmbh. Flipr tetra system, flipr calcium assay molecular devices. Flipr calcium assay kits provide the most comprehensive method for measuring changes of intracellular calcium for drug discovery and basic research and act as portfolio of calcium reagents. Assay kit is the key exception, as it enables high assay performance without a masking technology for quenchsensitive applications. The flipr calcium 5 assay kit dye is also expelled from the cell and no response is seen without probenecid. We have evaluated the flipr calcium 3 assay kit calcium 3, a new nowash fluorescence calcium indicator dye reagent, for the measurement of. This assay kit works by using a calcium fluorophore that is taken into the.